Technology

Platform Technology



Platform Technology


Repebody ™ technology
ArtBody ™ technology
ArtBody ™ ADC
01    Repebody ™ technology  |  Target specific monovalent binder
  • Repebody is an antibody-like protein scaffold composed of LRR (Leucine-rich repeat) modules that have the same antigen recognition function as human antibodies but have completely different structures. The Repebody scaffold has low immunogenicity, high structural stability at high temperature and pH variation, and has the advantage of being mass-produced using Escherichia coli. 

Repebody™


  • Scaffold: 8 LRR (leucine-rich repeat) modules - 
    Four variable sites/module
    ⇒ total 32 variable sites


  • Selection of a target-specific Repebody by a phage display and panning


  • Affinity modulation of a selected Repebody by   modular evolution and site-directed mutagenesis
02    ArtBody ™ technology  |  Target specific bivalent binder
  • ArtBody technology is a dual target binder designed to maximize tumor selectivity by combining Repebody units. It can be produced appropriate binding affinity into homo- or hetero form, and innovative technologies engineered for optimization to balance rapid uptake by target cells with anti-cancer efficacy. 

Repebody™

Monomer


  • M.W ~ 30 kDa
  • Binding affinity(KD) : nM ~ pM 


ArtBody™

Homo-dimer

  • M.W ~ 60 kDa 
  • Binding affinity(KD) : pM ~ fM (avidity effect)
    - Variable depending on the target density
    - Optimal combination of appropriate Repebodies

Hetero-dimer
03    ArtBody ™ ADC technology  |  Artificial antibody-drug conjugate
  • ArtBody scaffold that can be developed as an ADC was completed by introducing free cysteine that can conjugate drugs to ArtBody. ArtBody's DAR ranges from 1 to 4, so there is a wide range to choose from depending on the indication of the drug. 

ArtBody™ ADC technology

Homo-dimer


  • M.W ~ 60 kDa 
  • Binding affinity (KD) : pM ~ fM (avidity effect) 
    - Variable depending on the target density 
    - ArtBody with an optimal affinity for the highest selectivity toward tumor cells 


Hetero-dimer
  • ArtBody is designed with optimal binding affinity and selectively binds only to tumor cells expressing a certain level of TAA. ArtBody technology offers multiple benefits over traditional Abs. The safety profile is as in the figure below and ArtBody binds specifically to tumor cells while being safe against normal cells, optimal it a highly specific and safe drug. On the other hand, therapeutic antibodies are toxic to normal cells by binding to all cells expressing the target antigen with high binding affinity. 

상호명  주식회사 프로엔테라퓨틱스  |  대표자  이일한

전화번호  031-759-8788  |  팩스번호  031-759-8850

이메일  proen@proentherapeutics.com

판교지사  경기도 성남시 수정구 청계산로 686 반도아이비밸리 301호

대전본사  대전광역시 유성구 테크노4로 17 대덕비즈센터 B동 204호

Copyright 2021. ProEn Therapeutics Inc. All Rights Reserved.


ProEn Therapeutics Inc.

TEL  +(82)-31-759-8788  |  FAX  +(82)-31-759-8850  |  E-mail  proen@proentherapeutics.com

Address  Pangyo Branch #301, 686, Cheonggyesan-ro, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea

Copyright 2021. ProEn Therapeutics Inc. All Rights Reserved.